Efeito do exercí­cio de esteira rolante e da obestatina no perfil lipí­dico e ní­veis de malondialdeí­do sérico (MDA) em modelo de rato

  • Alireza Lotfi Young Researchers and Elite club, Ilkhchi Branch, Islamic Azad University, Ilkhchi, Iran
  • Sirvan Atashak Department of Exercise Physiology, Mahabad Branch, Islamic Azad University, Mahabad, Iran
Palavras-chave: Modelo animal, Perfil lipidico, Obestatina, Estresse oxidativo, Treinamento em esteira rolante

Resumo

Objective: The present study investigates the effect of intraperitoneal (IP) administered obestatin, short duration treadmill exercise on serum malondialdehyde (MDA) and lipid profile in the rat model. Materials and Methods: Forty-five rats weighing 100 ± 5g were selected and divided into five experimental groups; Group 1/control was normally maintained and received no solution or exercise included in obestatin. Group 2 (placebo) was treated with basal solution (50 ml of distilled water). Group 3 was referred to as the obestatin group, which underwent injection during the experiment with obestatin solution (10 microgram obestatin/mouse). Group 4 was known as the treadmill group that underwent the exercise protocol (15-day treadmill training) and group 5, known as the obestatin and treadmill (OT) group, underwent the obestatin injection and exercise protocol. . Exercise groups were trained for 3 weeks. Results: the treadmill group showed a decrease in total cholesterol (TC) concentration (67.67 mg/dl), compared to the control group and the obestatin group (79.00 and 78.33 mg/dl, respectively) . As with the CT level, triglyceride and HDL-cholesterol (HDL-C) decreased in the treadmill group (triglyceride: 70.66 mg/dl compared to 87.67 mg/dl in the control and HDL- C: 46.10 mg/dl compared to 57.33 mg/dl in control; p < 0.05). In addition, the MDA level decreased in the treadmill and treadmill-obestatin groups. Conclusion: Short-term treadmill exercise significantly reduced plasma lipid profile and MDA levels in the rat model. However, obestatin administration had no significant effect on lipid profile and MDA. Furthermore, administration of obestatin in combination with treadmill exercise had no synergistic effect on plasma lipids, MDA level and total protein in rats.

Referências

-Aragno, M.; and collaborators. Obestatininduced recovery of myocardial dysfunction in type 1 diabetic rats: underlying mechanisms. Cardiovascular Diabetology. Vol. 11. p. 129-140. 2012. DOI:10.1186/1475-2840-11-129

-Askew, E.W.; Hecker, A.L. Adipose tissue cell size and lipolysis in the rat: response to exercise intensity and food restriction. Journal of Nutrition. Vol. 106. Núm. 9. p. 1351-1360. 1976. DOI: 10.1093/jn/106.9.1351

-Badalzadeh, R.; Shaghaghi, M.; Mohammadi, M.; Dehghan, G.; Mohammadi, Z. (2014). The effect of cinnamon extract andlong-term aerobic training on heart function, biochemical alterations and lipid profile following exhaustive exercise in male rats. Advanced Pharmaceutical Bulletin. Vol. 4. Suppl. 2. p. 515-520. DOI: 10.5681/apb.2014.076

-Brooks, S.V.; Vasilaki, A.; Larkin, L.M.; McArdle, A.; Jackson, M.J. Repeated bouts of aerobic exercise lead to reductions in skeletal muscle free radical generation and nuclear factor kappaB activation. The Journal of Physiology. Vol. 586. p. 3979-3990. 2008. DOI: 10.1113/jphysiol.2008.155382

-Broom, D.R.; Batterham, R.L.; King, J.A.; Stensel, D.J. Influence of resistance and aerobic exercise on hunger, circulating levels of acylated ghrelin, and peptide YY in healthy males. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. Vol. 296. p. R29-35. 2009. DOI: 10.1152/ajpregu.90706.2008.

-Carpéné, C.; Les, F.; Estève, D.; Galitzky, J. Short-term effects of obestatin on hexose uptake and triacylglycerol breakdown in human subcutaneous adipocytes. World Journal of Diabetes. Vol. 9. Num. 1. p. 25-32. 2018. DOI:10.4239/wjd.v9.i1.25

-Cechetti, F.; Worm, P.V.; Elsner, V.R.; Bertoldi, K.; Sanches, E.; Ben, J.; Siqueira, I.R.; Netto, C.A. Forced treadmill exercise prevents oxidative stress and memory deficits following chronic cerebral hypoperfusion in the rat. Neurobiology of Learning and Memory. Vol. 97. Num. 1. p. 90-96. 2012. DOI: 10.1016/j.nlm.2011.09.008.

-Coll-Risco, I.; Aparicio, V.A.; Nebot, E.; Camiletti-Moirón, D.; Martínez, R.; Kapravelou, G.; López-Jurado, M.; Porres, J.M.; Aranda, P. Effects of interval aerobic training combined with strength exercise on body composition, glycaemic and lipid profile and aerobic capacity of obese rats. Journal of Sports Sciences, Vol. 34. Num. 15. p. 1452-1460. 2015. DOI: 10.1080/02640414.2015.1119296.

-Dehghan, G.; Shaghaghi, M.; Jafari, A.; Mohammadi, M.; Badalzadeh, R. Effect of endurance training and cinnamon supplementation on post-exercise oxidative responses in rats. Molecular Biology Research Communications. Vol. 3. Num. 4. p. 269-281. 2014. DOI: 10.22099/mbrc.2014.2723

-Diaz, K.M.; Feairheller, D.L.; Sturgeon, K.M.; Williamson, S.T.; Brown, M.D. Oxidative stress response to short duration bout of submaximal aerobic exercise in healthy young adults. International Journal of Exercise Science. Vol. 4. Num. 4. p. 247-256. 2011.Available: <https://digitalcommons.wku.edu/cgi/viewcontent.cgi?article=1333&context=ijes>

-Elahi, M.; Motoi, Y.; Matsumoto, S. Short-term treadmill exercise increased oxidative stress and tau insolubility in tauopathy model mice. Juntendo Medical Journal. Vol. 62. p. 76-76. 2016. DOI: 10.14789/jmj.62.s76.

-Erşahinm, M.; Ozsavci, D.; Sener, A.; Ozakpınar, O.B.; Toklu, H.Z.; Akakin, D.; Sener, G.; Yeğen, B.Ç. Obestatinalleviates subarachnoid haemorrhage-induced oxidative injury in rats via its anti-apoptotic and antioxidant effects. Brain Injury. Vol. 27. Num. 10. p. 1181-1189. 2013. DOI: 10.3109/02699052.2013.804199.

-Gargantini, E.; Grande, C.; Trovato, L.; Ghigo, E.; Granata, R. The role of obestatin in glucose and lipid metabolism. Hormone and Metabolic Research. Vol. 45. Num. 13. p. 1002-1008. 2013. DOI: 10.1055/s-0033-1351325.

-Gesmundo, I.; Gallo, D.; Favaro, E.; Ghigo, E.; Granata, R. Obestatin: a new metabolic player in the pancreas and white adipose tissue. International Union of Biochemistry and Molecular Biology. Vol. 65. p. 976-982. 2013. DOI: 10.1002/iub.1226

-Ghanbari-Niaki, A.; Behzad Khameslo, M.S.; Tayebi, M. Effect of pyramidal training on plasma lipid profile and fibrinogen, and blood viscosity of untrained young men. Annals of Applied Sport Science. Vol. 1. Num. 3. p. 47-56. 2013. Available: <http://aassjournal.com/article-1-83-en.pdf>

-Ghanbari-Niaki, A.; Jafari, A.M.; Moradi, R.R. Kraemer Short-, moderate-, and long-term treadmill training protocols reduce plasma, fundus, but not small intestine ghrelin concentrations in male rats. Journalof Endocrinological Investigation. Vol. 34. Num. 6. p. 439-343. 2011. DOI: 10.3275/7437.

-Ghanbari-Niaki, A.; Saghebjoo, M.; Rahbarizadeh, F. A single circuit-resistance exercise has no effect on plasma obestatin levels in female college students. Peptides. Vol. 29. p. 487-490. 2008. DOI: 10.1016/j.peptides.2007.11.002

-Greene, N.P.; Martin, S.E.; Crouse, S.F. Acute exercise and training alter blood lipid and lipoprotein profiles differently in overweight and obese men and women. Obesity. Vol. 20. Num. 8. p. 1618-1627. 2012. DOI: 10.1038/oby.2012.65.

-Guo, Z.F.; Zheng, X.; Qin, Y.W.; Hu, J.Q.; Chen, S.P.; Zhang, Z. Circulating preprandial ghrelin to obestatin ratio is increased in human obesity. Journal of Clinical Endocrinology and Metabolism. Vol. 92. p. 1875-1880. 2007. DOI:10.1210/jc.2006-2306

-Huang, C.J.; McAllister, M.J.; Slusher, A.L.; Webb, H.E.; Mock, J.T.; Acevedo, E.O. Obesity-related oxidative stress: the impact of physical activity and diet manipulation. Sports Medicine-Open. Vol. 1. Num. 1. p. 32. 2015. DOI: 10.1186/s40798-015-0031-y.

-Huertas, J.R.; Al Fazazi, S.; Hidalgo-Gutierrez, A.; López, L.C.; Casuso, R.A. Antioxidant effect of exercise: Exploring the role of the mitochondrial complex I super assembly. Redox Biology. Vol. 13. p. 477-481. 2017.

-Koyuncuoğlu, T.; Vızdıklar, C.; Üren, D.; Yılmaz, H.; Yıldırım, Ç.; Atal, S.S.; Akakın, D.; Kervancıoğlu Demirci, E.;Yüksel, M.; Yeğen, B.Ç. Obestatin improves oxidative brain damage and memory dysfunction in rats induced with an epileptic seizure. Peptides. Vol. 90. p. 37-47. 2017. DOI10.1016/j.peptides.2017.02.005.

-Lee, J.C.I.Y.; Kim, Y.; Son, S.K.; Byeon, D.H.; Yoon, J.S.; Son, H.S.; Song, W.; Song, J.K.; Seong, M.; Moon, H. Evaluation of treadmill exercise effect on muscular lipid profiles of diabetic fatty rats by nanoflow liquid chromatography-tandem mass spectrometry. Scientific Report, 6: ID:29617. 2016. DOI: 10.1038/srep29617

-Lotfi, A.; Atashak, S.; Narimani-Rad, M. Influence of obestatin and short-term treadmill exercise on plasma acyl-ghrelin and body weight in wistar Rat. Zahedan Journal of Research in Medical Sciences. Vol. 18. Num. 11. p. e4987. 2016. DOI: 10.17795/zjrms-4987.

-Miegueu, P.; St Pierre, D.; Broglio, F.; Cianflonem, K. Effect of desacyl ghrelin, obestatin and related peptides on triglyceride storage, metabolism and GHSR signaling in 3T3-L1 adipocytes. Journal of Cellular Biochemistry. Vol. 112. Num. 2. p. 704-714. 2011. DOI: 10.1002/jcb.22983

-Mokdad, A.H.; Marks, J.S.; Stroup, D.F.; Gerberding, J.L. Actual causes of death in the United States, 2000. JAMA. Vol. 291. Num. 10. p. 1238-1245. 2004. DOI: 10.1001/jama.291.10.1238.

-Olleveant, N.A. Information point: Tukey Multiple Comparison test. Journal of Clinical Nursing. Vol. 8. p. 299-304. 1999. Available: <http://www.blackwellpublishing.com/specialarticles/jcn_8_304.pdf>

-Park, W.H.; Oh, Y.J.; Kim, G.Y.; Kim, S.E.; Paik, K.H.; Kim, A.H.; Chu, S.H.; Kwon, E.K.; Kim, S.W.; Jin, D.K. Obestatinis not elevated or correlated with insulin in children with Prader–Willy syndrome. Journal of Clinical Endocrinology and Metabolism. Vol. 92. p. 229-234. 2007. Available: <https://doi.org/10.1210/jc.2006-0754>

-Quiles, J.L.; Huertas, J.R.; Mañas, M.; Ochoa, J.J.; Battino, M.; Mataix, J. Oxidative stress induced by exercise and dietary fat modulates the coenzyme Q and vitamin A balance between plasma and mitochondria. International Journal for Vitamin and Nutrition Research. Vol. 69. p. 243-249. 1999. DOI:10.1024/0300-9831.69.4.243

-Razzaghy-Azar, M.; Nourbakhsh, M.; Pourmoteabed, A.; Nourbakhsh, M.; Ilbeigi, D.; Khosravi, M. An evaluation of acylated ghrelin and obestatinlevels in childhood obesity and their association with insulin resistance, metabolic syndrome, and oxidative stress. Journal of Clinical Medicine. Vol. 5. Num. E61. 2016. DOI: 10.3390/jcm5070061.

-Sertié, R.A.L.; Christina Paulino, E.; Chakur Brum, P.; Andreotti, S.; Lima, F. B.; Negrão, C. E. Exercise Training and Caloric Restriction Reduce Adiposity Index and Hepatic Lipids in Obese Rats. Immunoendocrinology. Vol. 2.e1053. 2015.

-Taş, M.The effect of exercise treadmill of rat liver tissue. US-China Education Review A. Vol. 7. Num. 1. p. 58-62. 2017.

-Tiryaki-Sonmez, G.; Ozen, S.; Bugdayci, G.; Karli, U.; Ozen, G.; Cogalgil, S.; Schoenfeld, B.; Sozbir, K.; Aydin, K. Effect of exercise on appetite-regulating hormones in overweight women. Biology of Sport. Vol. 30. p. 75-80. 2013. DOI: 10.5604/20831862.1044220.

-Tiryaki-Sonmez, G.; Vatansever, S.; Olcucu, B.; Schoenfeld, B. Obesity, food intake and exercise: Relationship with ghrelin. Biomedical Human Kinetics. Vol. 7. Num. 116-124. 2015. DOI: 10.1515/bhk-2015-0018

-Troke, R.C.; Tan, T.M.; Bloom, S.R. The future role of gut hormones in the treatment of obesity. Therapeutic Advances in Chronic Disease. Vol. 5. Num. 1. p. 4-14. 2014. DOI: 10.1177/2040622313506730

-Vicennati, V.; Genghini, S.; De Iasio, R.; Pasqui, F.; Pagotto, U.; Pasquali, R. Circulating obestatinlevels and the ghrelin/obestatin ratio in obese women. European Journal of Endocrinology. Vol. 157. Num. 3. p.295-301. 2007. DOI:10.1530/EJE-07-0059

-Zhang, J.V.; Ren, P.G.; Avsian-Kretchmer, O. Obestatin, a peptide encoded by the ghrelin gene, opposes ghrelin’s effects on food intake. Science. Vol. 310. Num. 5750. p. 996-999. 2005. DOI:10.1126/science.1117255

-Zhang, Q.; Dong, X.W.; Xia, J.Y.; Xu, K.Y.; Xu, Z.R. Obestatinplays beneficial role in cardiomyocyte injury induced by ischemia-reperfusion in vivo and in vitro. Medical Science Monitor. Vol. 23. p. 2127-2136. 2017. DOI: 10.12659/MSM.901361

Publicado
2019-09-12
Como Citar
Lotfi, A., & Atashak, S. (2019). Efeito do exercí­cio de esteira rolante e da obestatina no perfil lipí­dico e ní­veis de malondialdeí­do sérico (MDA) em modelo de rato. RBNE - Revista Brasileira De Nutrição Esportiva, 13(80), 498-504. Recuperado de https://www.rbne.com.br/index.php/rbne/article/view/1385
Edição
v. 13 n. 80 (2019)
Seção
Artigos Científicos - Original

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