Determinando os efeitos combinados de três semanas de consumo de suplemento de hormônio e uma sessão de exercício de resistência extrema nas taxas de expressão de genes myog e cd56 em células satélites
O objetivo do presente estudo foi determinar os efeitos combinados do consumo de suplemento HMB-FA e uma sessão de treinamento de resistência extrema sobre a taxa de ativação das células satélites de ratos machos maduros. Para este fim, uma vez realizados os estudos preliminares, oitenta ratos machos Sprague Dowley (grupo controle: 18 ratos; grupo HMB: 19 ratos; grupo exercício: 19 ratos; grupo exercício + HMB: 19 ratos) com oito semanas e peso de 200 ± 20 gramas foram selecionados para o estudo. Após o curso de familiarização em escadas, a ingestão diária de suplemento foi iniciada e mantida por três semanas. Uma vez que os ratos foram familiarizados com a escada e o suplemento foi recebido por três semanas, uma sessão de treinamento de resistência foi administrado. O protocolo do treinamento incluiu descer de uma escada com uma inclinação de 80% enquanto um peso de 100-110% 1RM foi anexado às caudas dos ratos. Os resultados da extração de RNA e imuno-histoquímica mostraram que as três intervenções experimentais resultaram em um aumento na expressão dos genes MyoG e CD56 no tríceps. O ponto importante, no entanto, foi que a intervenção mais efetiva foi a intervenção exercício + HMB. As quantidades de genes CD56 e MyoG foram estatisticamente significativamente aumentadas nos grupos de HMB, Exercício e Exercício + HMB (p = 0,001). De acordo com os resultados obtidos, o consumo de suplemento de HMB em combinação com a efetivação de exercícios excêntricos pode desempenhar um papel efetivo na ativação, proliferação de propagação e diferenciação de células satélites. De fato, atletas e militares que em alguns pontos se envolvem em atividades físicas pesadas e usam treinamento preparatório extremo para maximizar sua prontidão física podem usar suplemento HMB simultaneamente com seu treinamento e obter resultados ainda mais eficientes.
Determining the combined effects of three weeks of consumption of HMB supplement and one session of extreme eccentric resistance exercise on the expression rates of myoG and CD56 genes in satellite cells
The purpose of the present study was to determine the combined effects of consumption of HMB-FA supplement and one session of extreme Eccentric resistance training on the rate of activation of the satellite cells of mature male rats. To this end, once the preliminary studies were done, eighty Sprague Dowley male rats (Control group: 18 rats; HMB group: 19 rats; Exercise group: 19 rats; Exercise + HMB group: 19 rats) aged eight weeks and weighting 200 ± 20 grams were selected for the study. After the course of familiarization on ladders, the daily intake of supplement was started and sustained for three weeks. Once the rats were familiarized with the ladder and the supplement was taken in for three weeks, a session of Eccentric resistance training was administered. The protocol of the training included descending from a ladder with a slope of 80% while a 100-110% 1RM weight was attached to the rats' tails. Results of extraction of RNA and Immunohistochemistry showed that the entire three experimental interventions have resulted in an increase in the expression of the MyoG and CD56 genes in the triceps. The important point however, was that the most effective intervention was the exercise + HMB intervention. The amounts of CD56 and MyoG genes have been statistically significantly increased in the groups of HMB, Exercise and, Exercise + HMB (p= 0.001). According to the obtained results, consumption of HMB supplement in combination with effectuation of Eccentric exercises can play an effective role in the activation, propagation proliferation and differentiation of satellite cells. In fact, athletes and military men whom at some points get involved in heavy physical activities and use extreme Eccentric preparatory training in order to maximize their physical readiness can use HMB supplement simultaneously with their training and get even more efficient results.
Texto completo:PDF (English)
-Alway, S.E.; and collaborators. β-Hydroxy-β-methylbutyrate (HMB) enhances the proliferation of satellite cells in fast muscles of aged rats during recovery from disuse atrophy. Experimental gerontology. Vol. 48. Num. 9. p. 973-984. 2013.
-Aversa, Z.; and collaborators. β-Hydroxy-β-methylbutyrate (HMB) prevents dexamethasone-induced myotube atrophy. Biochemical and biophysical research communications. Vol. 423. Num. 4. p. 739-743. 2012.
-Babcock, L.; and collaborators. Concurrent aerobic exercise interferes with the satellite cell response to acute resistance exercise. American Journal of Physiology-Regulatory, Integrative and Comparative Physiology. Vol. 302. Num. 12. p. R1458-R1465. 2012.
-Baxter, J.H.; and collaborators. Direct determination of β-hydroxy-β-methylbutyrate (HMB) in liquid nutritional products. Food Analytical Methods. Vol. 4. Num. 3. p. 341-346. 2011.
-Begue, G.; and collaborators. Early Activation of Rat Skeletal Muscle IL-6/STAT1/STAT3 Dependent Gene Expression in Resistance Exercise Linked to Hypertrophy. PloS one. Vol. 8. Num. 2. p. e57141. 2013.
-Burd, N.A.; and collaborators. Low-load high volume resistance exercise stimulates muscle protein synthesis more than high-load low volume resistance exercise in young men. PloS one. Vol. 5. Num. 8. p. e12033. 2010.
-Cermak, N.M.; and collaborators. Eccentric exercise increases satellite cell content in type II muscle fibers. Med Sci Sports Exerc. Vol. 45. Num. 2. p. 230-237. 2013.
-Crameri, R.M.; and collaborators. Changes in satellite cells in human skeletal muscle after a single bout of high intensity exercise. The Journal of physiology. Vol. 558. Num. 1. p. 333-340. 2004.
-Dreyer, H.C.; and collaborators. Satellite cell numbers in young and older men 24 hours after eccentric exercise. Muscle & nerve. Vol. 33. Num. 2. p. 242-253. 2006.
Fitschen, P.J.; and collaborators. Efficacy of β-hydroxy-β-methylbutyrate supplementation in elderly and clinical populations. Nutrition, 2012.
-Fry, C.S.; and collaborators. Fibre type‐specific satellite cell response to aerobic training in sedentary adults. The Journal of physiology. Vol. 592. Num. 12. p. 2625-2635. 2014.
-Fuller, J.C.; and collaborators. Comparison of availability and plasma clearance rates of β-hydroxy-β-methylbutyrate delivery in the free acid and calcium salt forms. British Journal of Nutrition. Vol. 114. Num. 9. p. 1403-1409. 2015.
-Heinemeier, K.M.; and collaborators. Short-term strength training and the expression of myostatin and IGF-I isoforms in rat muscle and tendon: differential effects of specific contraction types. Journal of Applied Physiology. Vol. 102. Num. 2. p. 573-581. 2007.
-Hoffman, J.R.; and collaborators. β-Hydroxy-β-methylbutyrate attenuates cytokine response during sustained military training. Nutrition research. Vol. 36. Num. 6. p. 553-563. 2016.
-Hyldahl, R.D.; and collaborators. Satellite Cell Response to a Repeated Bout of Eccentric Contractions in Human Skeletal Muscle: 2386 Board# 91 May 30, 9. Medicine & Science in Sports & Exercise. Vol. 46. Num. 5S. p. 640. 2014.
-Kadi, F.; and collaborators. The effects of heavy resistance training and detraining on satellite cells in human skeletal muscles. The Journal of physiology. Vol. 558. Num. 3. p. 1005-1012. 2004.
-Kim, J.-S.; and collaborators. β-hydroxy-β-methylbutyrate did not enhance high intensity resistance training-induced improvements in myofiber dimensions and myogenic capacity in aged female rats. Molecules and cells. Vol. 34. Num. 5. p. 439-448. 2012.
-Kornasio, R.; and collaborators. β-hydroxy-β-methylbutyrate (HMB) stimulates myogenic cell proliferation, differentiation and survival via the MAPK/ERK and PI3K/Akt pathways. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research. Vol. 1793. Num. 5. p. p. 755-763. 2009.
-Kurosaka, M.; and collaborators. Satellite cell pool enhancement in rat plantaris muscle by endurance training depends on intensity rather than duration. Acta Physiologica. Vol. 205. Num. 1. p. 159-166. 2012.
-Lieberman, H.R.; and collaborators. Severe decrements in cognition function and mood induced by sleep loss, heat, dehydration, and undernutrition during simulated combat. Biological psychiatry. Vol. 57. Num. 4. p. 422-429. 2005.
-Lima-Soares, F.; and collaborators. HMB Supplementation: Clinical and Performance-Related Effects and Mechanisms of Action, in Sustained Energy for Enhanced Human Functions and Activity, Elsevier. p. 363-381. 2018.
-MacDougall, D., Morphological changes in human skeletal muscle following strength training and immobilization. Human Muscle and Power, 1986.
-Maughan, R.J.; Burke, L. M.; Practical nutritional recommendations for the athlete. Nestle Nutr Inst Workshop Ser. Vol. 69. p. 131-49. 2011.
-McKay, B.R.; and collaborators. Satellite cell number and cell cycle kinetics in response to acute myotrauma in humans: immunohistochemistry versus flow cytometry. The Journal of physiology. Vol. 588. Num. 17. p. 3307-3320. 2010.
-McKay, B.R.; and collaborators. Myostatin is associated with age-related human muscle stem cell dysfunction. The FASEB Journal. Vol. 26. Num. 6. p. 2509-2521. 2012.
-Munroe, M.; and collaborators. Impact of β-hydroxy β-methylbutyrate (HMB) on age-related functional deficits in mice. Experimental gerontology. Vol. 87. p. 57-66. 2017.
-Nederveen, J.; and collaborators. The effect of exercise mode on the acute response of satellite cells in old men. Acta Physiologica. Vol. 215. Num. 4. p. 177-190. 2015.
-Nielsen, J.L.; and collaborators. Proliferation of myogenic stem cells in human skeletal muscle in response to low load resistance training with blood flow restriction. The Journal of physiology. Vol. 590. Num. 17. p. 4351-4361. 2012.
-O'Reilly, C.; and collaborators. Hepatocyte growth factor (HGF) and the satellite cell response following muscle lengthening contractions in humans. Muscle & nerve. Vol. 38. Num. 5. p. 1434-1442. 2008.
-Parise, G., McKinnell, I.W.; Rudnicki, M. A. Muscle satellite cell and atypical myogenic progenitor response following exercise. Muscle & nerve. Vol. 37. Num. 5. p. 611-619. 2008.
-Smith, H.K.; and collaborators. Exercise-enhanced satellite cell proliferation and new myonuclear accretion in rat skeletal muscle. Journal of Applied Physiology. Vol. 90. Num. 4. p. 1407-1414. 2001.
-Snijders, T.; and collaborators. Continuous endurance‐type exercise training does not modulate satellite cell content in obese type 2 diabetes patients. Muscle & nerve. Vol. 43. Num. 3. p. 393-401. 2011.
-Snijders, T.; and collaborators. The skeletal muscle satellite cell response to a single bout of resistance-type exercise is delayed with aging in men. Age. Vol. 36. Num. 4. p. 9699. 2014.
-Sukho, L.; Roger, F. Resistance training, muscle mass and function in the rats. Journal of Exercise Physiology. Vol. 62. p. 80-87. 2003.
-Vallejo, J.; and collaborators. Cellular and physiological effects of dietary supplementation with β-hydroxy-β-methylbutyrate (HMB) and β-alanine in late middle-aged mice. PloS one. Vol. 11. Num. 3. p. e0150066. 2016.
-Verdijk, L.B.; and collaborators. Skeletal muscle hypertrophy following resistance training is accompanied by a fiber type specific increase in satellite cell content in elderly men. The Journals of Gerontology Series A: Biological Sciences and Medical Sciences. Vol. 64. Num. 3. p. 332-339. 2009.
-Verney, J.; and collaborators. Effects of combined lower body endurance and upper body resistance training on the satellite cell pool in elderly subjects. Muscle & nerve. Vol. 38. Num. 3. p. 1147-1154. 2008.
-Wernbom, M.A.; and collaborators. Acute low-load resistance exercise with and without blood flow restriction increased protein signalling and number of satellite cells in human skeletal muscle. European journal of applied physiology. Vol. 113. Num. 12. p. 2953-2965. 2013.
- Não há apontamentos.
Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons
RBNE - Revista Brasileira de Nutrição Esportiva
IBPEFEX - Instituto Brasileiro de Pesquisa e Ensino em Fisiologia do Exercício
Editor-Chefe: Francisco Navarro. E-mail para contato: aqui