Ergogenic effect of supplementation of pump extract (Cucurbita moschata) in rats
Abstract
The consumption of biological compounds has been associated with the reduction of oxidative stress, maintenance of muscular recovery and maintenance of energy, thus increasing ergogenesis during intensive exercises. Vegetables contain phytochemicals that confer reduction of oxidative stress, improve sports performance, increase strength and endurance. The pumpkin (Cucurbita moschata) has received considerable attention in recent years due to the nutrition and health benefits attributed to bioactive compounds obtained from its fruit. This is an experimental study conducted with 14 Wistar rats (Rattus norvegicus). The animals were divided into two groups, a control group (CG, n = 6) receiving distilled water, and an experimental group (GE, n = 8), who received the ethanolic extract of the pumpkin (250mg / kg) by gavage during 21 days. The anti-fatigue effect and performance were assessed by means of exhaustive swimming exercise, as well as plasma lactate and glucose levels. At the end of the experiment, the animals were submitted to euthanasia, blood collection and removal of the liver. There was no significant difference between the groups in relation to the time of exhaustion and biochemical parameters evaluated. The consumption of the extract did not cause liver damage and had no impact on the body mass of the animals. Further studies with longer treatment times are needed to better define pumpkin ergogenesis in reducing fatigue and improving physical performance.
References
-Ambrósio, C.L.B.; Campos, C.S.F. Carotenóides como alternativa contra a hipovitaminose. A. Rev. Nutr. Vol.19. Num.2. p. 233-243. 2006.
-Attard, E.; Martinoli, M. G. Cucurbitacin E. An experimental lead triterpenoid with anticancer, immunomodulatory and novel effects against degenerative diseases. A mini review. cur top med chem. Hilversum. Vol. 15.p. 1708-1713. 2015.
-Bernardis, L.L.; Patterson, B.D. Correlation between 'Lee index' and carcass fat content in weanling and adult female rats with hypothalamic lesions. J. Endocrinol. Vol. 40. Núm. 8. p. 527. 1968.
-Biotfmusp. Biotério Central da Universidade de Medicina de São Paulo. 2009.
-Castelhano, C. The impact of light, noise, cage cleaning and in-house transport on welfare and stress of laboratory rats. Laboratory Animals. Vol. 43. Num. 4. p. 311-327. 2009.
-Cavalcanti. H. Resposta da variabilidade da frequência cardíaca e glicemia durante o exercício incremental. Brazilian journal of biomotricity. Vol. 4. Num. 4. p. 256-265. 2010.
-Chaput, J.P.; Tremblay, A. obesity at an early age and its impact on child. Encyclopedia on early childhood development. 2012.
-Draeger, C.L.; Naves, A.; Marques, N. Controversies of antioxidant vitamins supplementation in exercise: ergogenic or ergolytic effects in humans?J. Int. Soc. Sports Nutr. Vol. 11. Num. 1. p. 4. 2014.
-Hadad, N.; Levy, R. The synergistic anti-inflammatory effects of lycopene, lutein, β-carotene, and carnosic acid combinations via redox-based inhibition of NF-κB signaling. Free Radic. Biol. Med. Vol. 53. p. 381-1391. 2012.
-Khanna, G.L.; Manna, I. Supplementary effect of carbohydrate-electrolyte drink on sports performance, lactate removal & cardiovascular response of athletes. Indian J. Med. Res. Vol. 9. p. 121-665. 2005.
-Lee, J.; Kim, D.; Choi, J.; Choi, H.; Ryu, J.H.; Jeong, J.; Park, E.J.; Kim, S.H.; Kim, S. Dehydrodiconiferyl alcohol isolated from Cucurbita moschata shows anti-adipogenic and anti-lipogenic effects in 3T3-L1 cells and primary mouse embryonic fibroblasts. J. Biol. Chem. Vol. 287. p. 8839-8851. 2012.
-Martins, A. Bioactivephenolic compounds: Production and extraction by solidstate fermentation. A review. Biotechnology Advances. Vol. 29. p. 365-373. 2011.
-Miró, M. Cucurbitacins and their pharmacological effects. Phytother Res. Vol. 9. p. 159-168. 1995.
-Moura, M.C.; Queiróz, M. A; Gutman, S. M.; Silva, S. L.; Recio, M.C., Anti-inflammatory activity of two cucurbitacins isolated from Cayaponia tayuya roots. Planta Med, Stuttgart, Vol. 70. p. 414-420. 2004.
-Mueller, L.; Boehm, V. Antioxidant activity of β-carotene compounds in different in vitro assays. Molecules. Vol. 16. p.1055–1069. 2011.
-Petry E.; Alvarenga M.l.; Cruzat V.F.; Tirapegui J. Exercício físico e estresse oxidativo: mecanismos e efeitos. R. bras. ci. e mov. Vol. 18. Num. 4. p. 90-99. 2010.
-Ristow, K.; Zarse, A.; Oberbach, N.; Klöting, M.; Birringer, M.; Kiehntopf, M.; Stumvoll, C.R.; Kahn, M.; Blüher. Antioxidants prevent health-promoting effects of physical exercise in humans Proc. Natl. Acad. Sci. USA, Vol. 106. p. 8665-8670. 2009.
-Sahlin, K.; Shabalina, I.G.; Mattsson, C.M.; Bakkman, L.; Fernstrom, E.B.; Tonkonogi, M. Ultraendurance exercício aumenta a produção de espécies reativas de oxigênio em mitocôndrias isoladas do músculo esquelético humano.J. Appl. Physiol. Vol. 108. p. 780-787. 2010.
-Tanaka, T.; Shnimizu, M.; Moriwaki, H. Cancer chemoprevention by carotenoids. Molecules. Vol. 17. p. 3202-3242. 2012.
-Umigai, N.; Tanaka, J.; Tsuruma, K.; Shimazawa, M.; Hara, H. Crocetin, a carotenoid derivative, inhibits VEGF-induced angiogenesis via suppression of p38 phosphorylation. Curr. Neurovasc. Res. Vol. 9. p. 102-109. 2012.
-Wang, S.Y.; Huang, W.C.; Liu, C.C.; Wang, M.F.; Ho, C.S.; Huang, W.P.; Hou, C.C.; Chuang, H.L.; Huang, C.C.; Pumpkin (Cucurbita moschata) Fruit Extract Improves Physical Fatigue and Exercise Performance in Mice. Molecules. 2012.
-Wu, Cytotoxic and anti-HIV principles from the rhizomes of Begonia nantoensis. Chem Pharm Bull, Tokio, Vol. 52. p. 345-349. 2004.
-Wu, R.E.; Huang, W.C.; Liao, C.C.; Chang, Y.K.; Kan, N.W.; Huang, C.C. O resveratrol protege contra a fadiga física e melhora o desempenho do exercício em ratos. Molecules. Vol. 18. p. 4689-4702. 2013.
-Yfanti, C. Papel da suplementação de vitamina C e E na IL-6 em resposta ao treinamento. J. Appl. Physiol. Vol. 112. Num. 6. p. 990-1000.
Authors who publish in this journal agree to the following terms:
- Authors retain the copyright and grant the journal the right of first publication, with work simultaneously licensed under the Creative Commons Attribution License BY-NC which allows the sharing of the work with acknowledgment of the authorship of the work and initial publication in this journal.
- Authors are authorized to enter into additional contracts separately for non-exclusive distribution of the version of the work published in this journal (eg, publishing in institutional repository or book chapter), with acknowledgment of authorship and initial publication in this journal.
- Authors are allowed and encouraged to post and distribute their work online (eg, in institutional repositories or on their personal page) at any point before or during the editorial process, as this can bring about productive change as well as increase impact and impact. citation of published work (See The Effect of Free Access).
