Physicochemical analysis and comparison of the composition of whey protein and plant protein supplements
Abstract
The importance of proteins in the human diet is highlighted due to their essential role in physiological and immunological processes. Protein intake through dietary supplements is relevant in situations where regular food intake does not meet the daily nutrient requirements. This project evaluated the labeling accuracy of these supplements and how the protein and other nutrient levels compare to the values declared on the packaging, focusing on two categories: whey protein supplements and plant-based protein sources. Through exploratory and experimental research, proteins, carbohydrates, lipids, ash, and moisture were analyzed in nine supplement samples, divided into concentrate, isolate, and plant-based protein, following ANVISA regulations. Protein quantification was performed using the Kjeldahl Method, while lipids, carbohydrates, and ash were analyzed following specific protocols. The results indicated that 44.4% of the samples showed protein levels lower than those declared on the labels, but within the 20% variation limit established by RDC nº18 (2018). Regarding carbohydrates and lipids, 100% of the samples were non-compliant with the same regulation. These findings contribute to understanding the quality of supplements and their impact on food choices, promoting diversification in the consumption of these products. Moreover, they emphasize the need for rigorous monitoring to ensure compliance with regulations and protect consumers.
References
-Associação Brasileira de Empresas de Produtos Nutricionais (ABENUTRI). Resultado das análises PAM: produtos aprovados. ABENUTRI, setembro de 2022.
-AOAC. Association of Official Analytical Chemists. Official Methods of Analysis of AOAC International. 21st ed. Rockville, MD: AOAC International. 2019.
-Araújo, L.R.; Andreolo, J.; Silva, M.S. Utilização de suplemento alimentar e anabolizante por praticantes de musculação nas academias de Goiânia-GO. Revista Brasileira de Ciência e Movimento. Vol. 10. Num. 3. 2002.
-Bocquet, A.; Dupont, C.; Chouraqui, J. P.; Darmaun, D.; Feillet, F.; Frelut, M.L.; Girardet, J.P.; Hankard, R.; Lapillonne, A.; Rozé, J.C.; Simeoni, U.; Turck, D.; Briend, A. Efficacy and safety of hydrolyzed rice-protein formulas for the treatment of cow's milk protein allergy. Archives de Pédiatrie. Vol. 26. Num. 4. 2019. p. 238-246.
-Brasil. Agência Nacional de Vigilância Sanitária. Diretoria Colegiada. RDC n.º 243, de 26 de julho de 2018.
-Brasil. Agência Nacional de Vigilância Sanitária. Instrução Normativa nº 28, de 26 de julho de 2018. Estabelece as listas de constituintes, de limites de uso, de alegações e de rotulagem complementar dos suplementos alimentares. Diário Oficial da União: seção 1, Brasília-DF. 27 jul. 2018.
-Brasil. Agência Nacional de Vigilância Sanitária. Resolução RDC nº 18, de 27 de abril de 2010. Dispõe sobre o registro de medicamentos específicos. Diário Oficial da União: seção 1, Brasília-DF. 28 abr. 2010.
-Brasil. Resolução-RE nº 729, de 27 de fevereiro de 2014. Dispõe sobre normas e critérios técnicos para alimentos específicos. Diário Oficial da União: seção 1, Brasília-DF. 27 fev. 2014.
-Chang, C.H.; Lin, C.Y.; Lo, Y.L.; Lin, T.Y.; Hung, C.Y.; Hsieh, M.H.; Fang, Y.F.; Huang, H.Y.; Lin, S.M.; Lin, H.C. Higher protein intake may benefit in patients with prolonged mechanical ventilation. Frontiers in Nutrition. Vol. 11. 2024.
-Cruz-Jentoft, A.J.; Bahat, G.; Bauer, J.; Boirie, Y.; Bruyère, O.; Cederholm, T.; Cooper, C.; Landi, F.; Rolland, Y.; Sayer, A.A.; Schneider, S.M.; Sieber, C.C.; Topinkova, E.; Vandewoude, M.; Visser, M.; Zamboni, M. Sarcopenia: Revised European consensus on definition and diagnosis. Age Ageing. Vol. 0. 2018. p. 1-16.
-Devlin, T. M. Manual de bioquímica com correlações clínicas. 7 edição. 2011.
-Devries, M.C.; Phillips, S.M. Supplemental protein in support of muscle mass and health: advantage whey. Journal of Food Science. Vol. 80. Suppl 1. 2015. p. A8-A15.
-Domingues, L. Proteína e aumento da massa muscular em praticantes de exercícios resistidos. Londrina. Unopar. 2023.
-Draganidis, D.; Karagounis, L.G.; Athanailidis, I.; Chatzinikolaou, A.; Jamurtas, A.Z.; Fatouros, I.G. Inflammaging and skeletal muscle: Can protein intake make a difference? Journal of Nutrition. Vol. 146. 2016.
-Farias, C.S.; Stefani, G.P.; Schneider, C.D.; Lando, V.R. Análise de concentração de proteínas em diferentes tipos de suplementos proteicos nacionais. Revista Brasileira de Nutrição Esportiva. São Paulo. Vol. 13. Num. 81. 2019. p. 705-712.
-Gorissen, S.H.M.; Crombag, J.J.R.; Senden, J.M.G.; Waterval, W.A.H.; Bierau, J.; Verdijk, L. B.; van Loon, L.J.C. Protein content and amino acid composition of commercially available plant-based protein isolates. Amino Acids. Vol. 50. Num. 12. 2018. p. 1685-1695.
-Guillet, C.; Masgrau, A.; Boirie, Y. Is protein metabolism changed with obesity? Current Opinion in Clinical Nutrition & Metabolic Care. Vol. 14. 2011. p. 89-92.
-Lynch, H.; Johnston, C.; Wharton, C. Plant-based diets: Considerations for environmental impact, protein quality, and exercise performance. Nutrients. Vol. 10. 2018. p. 1841.
-Lyu, J.; Lim, J.Y.; Han, Y.; Na, K.; Jung, S.; Park, Y.J. Protein source associated with risk of metabolic syndrome in adults with low and adequate protein intake: A prospective cohort study of middle-aged and older adults. The Journal of Nutrition, Health and Aging. Vol. 28. 2024.
-Pal, S.; Radavelli-Bagatini, S. The effects of whey protein on cardiometabolic risk factors. Obesity Reviews. Vol. 14. 2013. p. 324-343.
-Pereira, R.F.; Lajolo, F.M.; Hirschbruch, M.D. Consumo de suplementos por alunos de academias de ginástica em São Paulo. Revista de Nutrição. Vol. 16. Num. 3. 2003.
-Philp, A.; Hargreaves, M.; Baar, K. More than a store: Regulatory roles for glycogen in skeletal muscle adaptation to exercise. American Journal of Physiology - Endocrinology and Metabolism. Vol. 302. 2012. p. 1343-1351.
-Phillips, S.M. Nutrient-rich meat proteins in offsetting age-related muscle loss. Meat Science. Vol. 92. 2012. p. 174-178.
-Putra, S.N.K.M.; Ishak, N.H.; Sarbon, N.M. Preparation and characterization of physicochemical properties of golden apple snail (Pomacea canaliculata) protein hydrolysate as affected by different proteases. Biocatalysis and Agricultural Biotechnology. Vol. 13. Num. 1. 2018. p. 123-128.
-Rego, P.C.S.; Andrade Júnior, F.P.S.; Souza, J.B.P. Determinação de proteínas totais em suplementos proteicos advindos do soro do leite. Journal of Medicine and Health Promotion. Vol. 5. Num. 2. 2020. p. 38-47.
-Rieu, I.; Balage, M.; Sornet, C.; Debras, E.; Ripes, S.; Rochon-Bonhomme, C.; Pouyet, C.; Grizard, J.; Dardevet, D. Increased availability of leucine with leucine-rich whey proteins improves postprandial muscle protein synthesis in aging rats. Nutrition. 2007. p. 323-331.
-Rizzoli, R.; Chevalley, T. Nutrition and osteoporosis prevention. Current Osteoporosis Reports. Vol. 22. 2024. p. 515-522.
-Sabaté, J.; Soret, S. Sustainability of plant-based diets: Back to the future. American Journal of Clinical Nutrition. Vol. 100. 2014. p. 476S-482S.
-Santos, E.V.B.; Oliveira, R.A.; Marinelli, P.S.; Machado, F.M.V.F. Quantificação de teores de proteínas totais em suplementos de whey protein concentrado. Revista Brasileira de Nutrição Esportiva, São Paulo. Vol. 17. Num. 104. 2023. p. 365-373.
-Volek, J.S.; Volk, B.M.; Gómez, A.L.; Kunces, L.J.; Kupchak, B.R.; Freidenreich, D.J.; Aristizabal, J.C.; Saenz, C.; Dunn-Lewis, C.; Ballard, K.D.; Quann, E.E.; Kawiecki, D.L.; Flanagan, S.D.; Comstock, B.A.; Fragala, M.S.; Earp, J.E.; Fernandez, M.L.; Bruno, R.S.; Ptolemy, A.S.; Kellogg, M.D.; Maresh, C.M.; Kraemer, W.J. Whey protein supplementation during resistance training augments lean body mass. Journal of the American College of Nutrition. Vol. 32. 2013. p. 122-135.
-Weng, S. I. In: Food and society. Trending foods and beverages. 2020. p. 305-321.
-Wróblewska, B.; Juśkiewicz, J.; Kroplewski, B.; Jurgoński, A.; Wasilewska, E.; Złotkowska, D.; Markiewicz, L. The effects of whey and soy proteins on growth performance, gastrointestinal digestion, and selected physiological responses in rats. Food Function, Vol. 9. 2018. p. 1500-1509.
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